ALUNBRIG® (brigatinib) Exhibits Broad Mutational Coverage and Activity in the CNS1

ALUNBRIG inhibits tumor proliferation and growth in animal models.1

How ALUNBRIG Works as an ALK Inhibitor to Treat ALK+ mNSCLC

ALUNBRIG Mechanism of Action (MOA)

Brigatinib molecular structure illustration.

 

  • ALUNBRIG is a tyrosine kinase inhibitor with in vitro activity at clinically achievable concentrations against multiple kinases, including ALK, ROS1, insulin-like growth factor-1 receptor (IGF-1R), and FLT-3 as well as EGFR deletion and point mutations1
  • ALUNBRIG inhibited ALK autophosphorylation and the activation of downstream signaling proteins1
    • Inhibited proteins: STAT3, AKT, ERK1/2, and S6 in in vitro and in vivo assays1
  • ALUNBRIG was shown to inhibit cell proliferation1
    • Affected the in vitro proliferation of cell lines expressing EML4-ALK and NPM-ALK fusion proteins1
  • ALUNBRIG inhibited tumor growth1
    • Demonstrated dose-dependent inhibition of EML4-ALK+ NSCLC xenograft growth in mice1
    • At clinically achievable concentrations (<500 nM), brigatinib inhibited the in vitro viability of cells expressing EML4-ALK and 17 mutant forms associated with resistance to ALK inhibitors including crizotinib, as well as EGFR-Del (E746-A750), ROS1-L2026M, FLT3-F691L, and FLT3-D835Y1

In Vitro: ALUNBRIG Inhibited 17 ALK Inhibitor-Resistant Mutations2

ALUNBRIG exhibited in vivo antitumor activity against 4 mutant forms of EML4-ALK, including the G1202R and L1196M mutants identified in NSCLC tumors in patients who had progressed on crizotinib.1

Comparison chart for 17 ALK inhibitor­resistant mutations inhibited by ALUNBRIG® in vitro.

ALUNBRIG Demonstrated In Vivo Antitumor Activity in CNS in Animal Models1

ALUNBRIG reduced tumor burden and prolonged survival in mice with an ALK-driven tumor cell line implanted intracranially.1

Preclinical activity does not necessarily correlate with clinical outcomes.

Explore systemic first-line efficacy findings for ALUNBRIG in ALK+ mNSCLC1

1L, first-line; ALK, anaplastic lymphoma kinase; ALK+ mNSCLC, anaplastic lymphoma kinase + metastatic non-small cell lung cancer; AKT, protein kinase B; APRN, Advanced Practice Registered Nurse; CNS, central nervous system; EGFR, epidermal growth factor receptor; EGFR-Del, epidermal growth factor receptor deletion; ERK1/2, extracellular signal-regulated kinase 1/2; EML4-ALK, echinoderm microtubule-associated protein-like 4 anaplastic lymphoma kinase; FLT-3, FMS‐like tyrosine kinase 3; FNP-C, IL, Family Nurse Practitioner Certified, Illinois; mNSCLC, metastatic NSCLC; MOA, mechanism of action; MSN, Master of Science in Nursing; NSCLC, non-small cell lung cancer; NPM-ALK, nucleolar protein nucleophosmin- anaplastic lymphoma kinase; ROS1, ROS proto-oncogene 1; S6, ribosomal protein S6; STAT3, signal transducer and activator of transcription 3.